Qualitative detection of muramic acid in normal mammalian tissues
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چکیده
منابع مشابه
Failure To detect muramic acid in normal rat tissues but detection in cerebrospinal fluids from patients with Pneumococcal meningitis.
Muramic acid serves as a marker for the presence of bacterial cell wall debris in mammalian tissues. There have been a number of controversial and sometimes conflicting results on assessing the levels of muramic acid in health and disease. The present report is the first to use the state-of-the art technique, gas chromatography-tandem mass spectrometry, to identify and quantify the levels of mu...
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1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides rema...
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While investigating the metabolism of ethanolamine by rat liver microsomes, we observed an incorporation of this amine into microsomal lipid that was dependent on the presence of a fatty acid as cosubstrate (1). It was shown that the product of the enzymatic reaction was the fatty acid amide of ethanolamine and that the reaction was synthetic, since little hydrolysis of added fatty acid amide c...
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In previous studies, we showed that peptidoglycan polysaccharides from anaerobic bacteria normally present in the human gut induced severe chronic joint inflammation in rats. Our hypothesis is that peptidoglycan from the gut flora is involved in perpetuation of idiopathic inflammation. However, in the literature, the presence of peptidoglycan or subunits like muramyl peptides in blood or tissue...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1984
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.43.3.937-941.1984